Method: Immunology Diagnostic Test
Principle: Indirect immunofluorescence tests (IIFT)
The BIOCHIP technology developed by EUROIMMUN allows unrivalled quality and variety in IIFT. Due to a special activation technique, cover glasses are modified in such way that very different substrates can be coated with highest adhesion. Cultured cells, tissue sections, antigen-expressing cells and purified antigens (antigen dots) are used as substrates. The cover glasses coated with biological substrates are cut mechanically into millimetre-sized fragments (BIOCHIPs). Many preparations with consistent, high quality can be obtained per tissue section, for cultured cell substrates even several thousands. The BIOCHIPs are fixed precisely on the test fields of specially developed plastic slides. When multiple BIOCHIPs coated with different substrates are arranged in one reaction field, antibodies against various organs or infectious agents can be investigated simultaneously. Such BIOCHIP mosaics allow the generation of comprehensive antibody profiles (multiplex) with minimal sample volumes or the reciprocal verification of the results on different substrates.

Advantages of EUROIMMUN IIFT
– Unrivalled standardisation of IIFT due to BIOCHIP technology with highest quality on millimetre-sized fragments
– Combination of BIOCHIPs in BIOCHIP Mosaics on one reaction field for multiplex testing
– Comprehensive product range for autoimmune and infection diagnostics based on BIOCHIPs with cultured cells, antigen dots (EUROPLUS), antigen-expressing cells and tissue sections
– Test kits containing ready-for-use reagents that are exchangeable between products with bar and colour codes for secure manual and automated processing
– Various test kit formats for different sample throughputs: slides with 5, 10 and 50 reaction fields
– Universal incubation schemes for uncomplicated combined processing of different parameters
– DataMatrix codes on all slides for complete traceability
– Brilliant fluorescence images due to incubation using TITERPLANE technology Fully Automated Analyzer & Semi-Automated Analyzer
IIFT Automated Analyzer & Fluorescence Microscope (cLED) ● IF Sprinter
● Sprinter XL
● EUROLabWorkstation IFA
● UNIQO 160
Microscope and Computer Aids Fully Automated Microscope (EUROStar and EUROPattern series)
● EUROPattern Microscope Live
● EUROStar IV Plus
● EUROPicture ● EUROPattern II

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